Fmoc-Ile-OH CAS 71989-23-6 Fmoc-L-Isoleucine Purity >99.0% (HPLC) Factory

Fmoc-L-Isoleucine (Fmoc-Ile-OH) (CAS: 71989-23-6) is an isoleucine derivative, is an unnatural amino acid used for peptidomimetics. Fmoc-L-isoleucine is an Nα-9-fluorenylmethoxycarbonyl-amino acid. 9-fluorenylmethoxycarbonyl is an important amino protecting group in peptide synthesis. Because it is a base-sensitive protecting group and is stable to the conditions used to remove common protecting groups, it has a unique role in polypeptide synthesis. Standard building block for routine solid phase peptide synthesis following the Fmoc-tactics.  
Fmoc-amino acids, used in peptide synthesis, used as an organic synthesis intermediate, pharmaceutical intermediate, biochemical reagent or chemical reagent.
Preparation L-Isoleucine (CAS: 73-32-5) 1.05g (0.008 mol) of solid was dissolved in 10% sodium carbonate solution, L-isoleucine solid was sufficiently dissolved by stirring. At 20~30℃, 9-fluorenylmethoxycarbonyl chloride (205g) dissolved in toluene (2~2.10) was added dropwise, 0.008 mol) solution, Dropwise adding 30~60 minutes, the end of the addition, at 20~30 C stirring 1~8 hours, add 30~200 ml of water dilution, with n-butyl acetate (80 ml) excess 9-fluorenylmethoxycarbonyl chloride was removed by extraction, the resulting aqueous phase was acidified with concentrated hydrochloric acid to PH = 0.5~3.5, then extracted with n-butyl acetate (80 ml), and the resulting oil phase was washed with water to remove hydrochloric acid, the oil phase was concentrated to remove the n-butyl acetate solvent, white crystals precipitated, filtered and dried to give the product Fmoc-L-Isoleucine 2.48g, yield 87.9%, Melting Point: 140.0~155.0℃.